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Abstract

BACKGROUND:

Human embryonic stem cells are derived from inner cell mass of the human blastocyst. The human embryonic stem cells are capable of the unlimited proliferation and maintain the undifferentiated state in long-term culturing in vitro, and can be differentiated into all kinds of cell types of the human body organizations.

OBJECTIVE:

To study the hematopoietic differentiation of human embryonic stem cells HuES17 cell line.

METHODS:

Human embryonic stem cells HuES17 were co-cultured with human foreskin fibroblast cells, and the human embryonic stem cells were induced to differentiate into hematopoietic stem cells by co-culturing with bone marrow stromal cells in mice (OP9 cells).

RESULTS AND CONCLUSION:

When human embryonic stem cells co-cultured with OP9 cells for 5-6 days, the OP9 cells began to aging, and soon died; the differentiation of human embryonic stem cells could be observed. However, with the OP9 cells death, the differentiated human embryonic stem cells were also dead, and could not induce the human embryonic stem cells to differentiate into hematopoietic stem cells. Human embryonic stem cells HuES17 cells line showed little inclination to differentiate into hematopoietic stem cells, or may not be induced to differentiate into hematopoietic stem cells.

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