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Abstract

BACKGROUND:

Recent researches have reported that bone marrow mesenchymal stem cells transplantation can be used for the treatment of acute kidney injury, and human amnion-derived mesenchymal stem cells have a good therapeutic effect in repairing other tissues and organs injury.

OBJECTIVE:

To investigate the effect of the human amnion-derived mesenchymal stem cells transplantation on cisplatin-induced acute kidney injury.

METHODS:

Human amnion-derived mesenchymal stem cells were isolated, cultured and identified in vitro. Male ICR mice were randomly divided into three groups: normal control group, model group, human amnion-derived mesenchymal stem cells group, and mices in the model group and human amnion-derived mesenchymal stem cells group were used to establish acute kidney injury models. In the human amnion-derived mesenchymal stem cells group, 0.3 mL human amnion-derived mesenchymal stem cells with the concentration of 1.0×1010/L were injected into the mice via tail vein at 8 days; mice in the model group and normal control group were injected with normal saline in the same dose.

RESULTS AND CONCLUSION:

At 17 days after the establishment of cisplatin-induced acute kidney injury model by human amnion-derived mesenchymal stem cells transplantation, renal function examination results demonstrated that compared with model group, the blood urea nitrogen and serum creatinine levels in human amnion-derived mesenchymal stem cells group were significantly increased, and reached to the levels in normal control group. Hematoxylin-eosin staining results showed the clear and complete renal organizational structure with rich tubules in the human amnion-derived mesenchymal stem cells group, the integrity and structure were similar to those of the mice in the normal control group, but the pathological changes of the human amnion-derived mesenchymal stem cells group were significantly improved when compared with model group. At 7 days after human amnion-derived mesenchymal stem cells transplantation, double labeling immunofluorescence results showed there was a large number of human amnion-derived mesenchymal stem cells colonization and distributionin in the nephridial tissue, a few MAB1281-FITC positive cells derived from human amnion-derived mesenchymal stem cells also found in the epithelium of kidney tubules. The human amnion-derived mesenchymal stem cells transplantation could promote the recovery of acute kidney injury.

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