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Abstract

BACKGROUND:

The multi-lineage differentiation potential, high amplification rate and accessibility of the dental pulp cells make them to become an attractive source of mesenchymal stem cells.

OBJECTIVE:

To observe the proliferation characteristics of rabbit dental pulp cells and to identify the cells surface marker.

METHODS:

The dental pulp cells were isolated and cultured in vitro, then the cells were cultured to the third generation by enzymatic digestion method to observe the morphological changes, calculate the cell survival rate, test the cell clone forming rate, measure the cell growth curve and the cell proliferation cycle, and identify the cell surface markers.

RESULTS AND CONCLUSION:

The enzymatic digestion method could rapidly harvest various generations of rabbit dental pulp cells. The proportion of the cell survival rate was 94.7%:95.8%:95.2%:95.3% from the third generation to the sixth generation. The cells clone forming rate was 18/2 000; the cell growth curve was in line with the characteristics of mesenchymal cells, and the cell cycle rate of G0 and G1 was more than 80%; cell multiplication cycle DNA purity more than 80 %. Immunocytochemistry staining showed the positive expression of vimentin, CD44, osteonectin and dentin sialoprotein. It indicates that the dental pulp stem cells can be isolated from the rabbit dental pulp cells and effectively proliferated in vitro.

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