The colonization tracer technology after stem cells transplantation includes magnetic probe labeling, fluorescent tracer method, lentivirus infections fluorescent labeling and Y chromosome in situ hybridization technology, and each method has its own disadvantages and advantages.OBJECTIVE:
To observe the transplantation conditions of hepatic stem cells (hepatic oval cells) in the liver graft by Y-chromosome in situ hybridization technology.METHODS:
Rat orthotropic liver transplantation models were established by using Kamada two-cuff technique. Female DA rats served as donors, and 60 female Lewis rats were used as recipients. Hepatic oval cells was originated from male Lewis rats and implanted to the liver graft during liver transplantation. Y-chromosome in situ hybridization technology was used to detect hepatic stem cells transplantation conditions in the liver graft.RESULTS AND CONCLUSION:
Hepatic oval cells and differentiated cells mainly distributed around central vein area and portal area in the liver graft. The transplantation conditions of recipient derived hepatic stem cells in the liver graft were effectively detected by Y-chromosome in situ hybridization technology.