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Abstract

BACKGROUND:

Platelet-rich plasma, when activated, could secret multiple growth factors which may promote the proliferation and differentiation of bone marrow-derived mesenchymal stem cells.

OBJECTIVE:

To explore the effect of activated autologous platelet-rich plasma on the chondrogenic differentiation of rabbit bone marrow-derived mesenchymal stem cells in vitro.

METHODS:

Rabbit bone marrow-derived mesenchymal stem cells were isolated using the whole bone marrow adherence method. Passage 3 bone marrow-derived mesenchymal stem cells were in vitro cultured with 10% activated autologous platelet-rich plasma and 10% fetal bovine serum separately. Chondrogenic differentiation of rabbit bone marrow-derived mesenchymal stem cells in vitro was observed.

RESULTS AND CONCLUSION:

Bone marrow-derived mesenchymal stem cells were successfully isolated and exhibited a long-shuttle-shaped appearance. Cells grew faster when passaged. Immunofluorescence staining showed that after induced by activated autologous platelet-rich plasma, bone marrow-derived mesenchymal stem cells exhibited type II collagen expression. Quantitative real-time PCR analysis showed that the expression of α1 chain of type II collagen and aggrecan mRNA was significantly up regulated in the bone marrow-derived mesenchymal stem cells induced by activated autologous platelet-rich plasma than in the cells induced by fetal bovine serum (P < 0.01). Our findings in this study suggested that activated autologous platelet-rich plasma can promote the chondrogenic differentiation of rabbit bone marrow-derived mesenchymal stem cells in vitro.

RESULTS AND CONCLUSION:

Wang SZ, Wang C, Rui YF. Effect of activated autologous platelet-rich plasma on chondrogenic differentiation of rabbit bone marrow-derived mesenchymal stem cells in vitro. Zhongguo Zuzhi Gongcheng Yanjiu. 2013;17(1):1-8.

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