Few reports have reported the effects of anesthetic with different ingredients and concentrations on the biological characteristics of adipocyte and human adipose-derived stem cells, and the negative impacts such as toxic reaction are still unclear.OBJECTIVE:
To investigate the effects of different anesthetic conditions on the proliferation and adipogenic differentiation of human adipose-derived stem cells in vitro in order to reduce anesthetic-induced damage and increase the availability of human adipose-derived stem cells.METHODS:
Human adipose-derived stem cells were isolated from the subcutaneous adipose tissue of healthy adults after liposuction, and primary culture and subculture of human adipose-derived stem cells were conducted. Passage 3 human adipose-derived stem cells were treated with 0.2 g/L of final concentration medium which containing lidocaine, ropivacaine hydrochloride and bupivacaine, respectively. A control group was designated. The damage condition, growth and proliferation of human adipose-derived stem cells were analyzed and the adipogenic differentiation ratio was compared.RESULTS AND CONCLUSION:
Compared with the control group, the lactate dehydrogenase level of cell supernatants in lidocaine group, ropivacaine group and bupivacaine group at all time points was significantly increased, the absorbance value was decreased, and the difference was significant (P < 0.05); furthermore, there was significant difference in the lactate dehydrogenase level of cell supernatants at all time points between lidocaine group and ropivacaine group or bupivacaine group (P < 0.05). There was no significant difference of adipogenic differentiation ratio of human adipose-derived stem cells between the four groups (P > 0.05). The medium containing lidocaine, ropivacaine hydrochloride and bupivacaine at 0.2 g/L final concentration exhibits the growth and proliferation of human adipose-derived stem cells, but it has no influence on the adipogenic differentiation of human adipose-derived stem cells.RESULTS AND CONCLUSION:
Wu LX, Li HM, Liu DL, Nan H. Effect of different anesthetic conditions on proliferation and adipogenic differentiation of human adipose-derived stem cells. Zhongguo Zuzhi Gongcheng Yanjiu. 2013;17(1):38-43.