The use of cloned human embryos gives rise to ethical issues and allows scientists to find alternative ways to reverse differentiation of cells into multi/pluripotent stem cells, which is known as reprogramming. The new method of reprogramming is the focus of attention.OBJECTIVE:
To investigate the current status of reprogramming and review the methods of somatic cell reprogramming into stem cells.METHODS:
Using the database of CNKI and Pubmed (1983-01/2006-12) to search the related articles about reprogramming. The retrieval words include reprogramming, method, somatic cell, stem cell, differentiation. Papers related reprogramming published recently or in high-impact journals were initially surveyed and 17 papers were included in the final analysis.RESULTS AND CONCLUSION:
The reprogramming of a pluripotent stem cell is the consequence of gradual reconstruction of cell structure, genetic change of chromatin, transcription expression and posttranscriptional control. Remodeling of targeted cells requires a stable status of reprogramming and a final reorientation into specific differentiation. Sufficient evidence demonstrates that cell identification is influenced by in vitro operation. The fate of reprogrammed cells needs further in vivo determination.RESULTS AND CONCLUSION:
Ruan GP, Wang JX, Yang XY, Song QQ, Yao X, Pang RQ, Pan XH. Research progress in reprogramming somatic cells into stem cells. Zhongguo Zuzhi Gongcheng Yanjiu. 2013;17(1):156-160.