Periodontal ligament stem cells are the adult stem cells in periodontal tissues, which possess the ability for strong proliferation, self-renewal and multipotent differentiation. These cells can promote the osteoblast differentiation of periodontal ligament stem cells and therefore assist in treatment of periodontal diseases.OBJECTIVE:
To investigate the effects of insulin-like growth factor-1 and fibroblast growth factor-2 on osteoblast differentiation of periodontal ligament stem cells.METHODS:
Human periodontal ligament stem cells were harvested from periodontal ligament tissue by collagenase digestion. After in vitro identification, amplification and hematoxylin-eosin staining, periodontal ligament stem cells were biologically detected by flow cytometry. Then these cells were induced with osteogenic induction medium, insulin-like growth factor-1 or fibroblast growth factor-2 for 7 and 14 days. Alkaline phosphatase staining, alkaline phosphatase activity detection and alizarin red staining were performed. In addition, real-time quantitative PCR method was performed to detect the expression of marker gene of osteogenic differentiation.RESULTS AND CONCLUSION:
Compared to the control group, alkaline phosphatase activity and calcified nodules were significantly greater, and Runx2, Alp, and col-1 mRNA expression was significantly higher, in the insulin-like growth factor-1 group. These findings suggest that insulin-like growth factor-1 and fibroblast growth factor-2, to different extents, promote osteogenic differentiation of periodontal ligament stem cells cultured in vitro.RESULTS AND CONCLUSION:
Song J, He JM, Li S, Yan XP, Lu H. Role of cytokines in osteoblast differentiation of periodontal ligament stem cells. Zhongguo Zuzhi Gongcheng Yanjiu. 2013;17(10):1821-1826.