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Abstract

BACKGROUND:

To date, most researchers use 5-azacytidine to induce the bone marrow mesenchymal stem cells to differentiate into cardiomyocytes, and there are certain adverse reactions which are difficult to be applied in the clinic. Chinese medicine Danshen is widely used in the clinical treatment of cardiovascular diseases, and its main chemical components are salvianolic acid B and tanshinone II A

OBJECTIVE:

To explore the effect of salvianolic acid B and tanshinone II A in inducing bone marrow mesenchymal stem cells to differentiate into cardiomyocyte-like cells.

METHODS:

Bone marrow mesenchymal stem cells of Sprague-Dawley rats were isolated and cultured from rat bone marrow, and then the second generation of bone marrow mesenchymal stem cells were induced by salvianolic acid B, tanshinone II A and their combination. The cells without inductor were considered as blank control group. After cultured for 3 days, the induction medium was removed and cultured with normal culture medium for 4 weeks.

RESULTS AND CONCLUSION:

Desmin, α-sarcomeric actin, cardiac troponin T and connexin 43 in the blank control group showed weakly positive or negative expression. Compared with the blank control group, the positive expressions of desmin, α-sarcomeric actin, cardiac troponin T and connexin 43 in salvianolic acid B group, tanshinone II A group and salvianolic acid B + tanshinone II A group were significantly increased, and the differences were significant (P < 0.01). The positive expression rate of the markers above was highest in the salvianolic acid B + tanshinone II A group. Fluorescent immunocytochemistry staining showed that the expression of desmin in the cells of induction groups was red and the expression of cardiac troponin T was green, while the part that overlaps was yellow. The results showed that salvianolic acid B and tanshinone II A may induce bone marrow mesenchymal stem cells to acquire cardiogenic phenotype separately, and the effect of combined induction is better than single ingredient.

RESULTS AND CONCLUSION:

Li X, Wang YL. Salvianolic acid B and tanshinone II A induce bone marrow mesenchymal stem cells to differentiate into cardiomyocyte-like cells. Zhongguo Zuzhi Gongcheng Yanjiu. 2013;17(10):1849-1855.

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