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Abstract

BACKGROUND:

Ginseng has a wide range of pharmacological activities, such as anti-inflammatory, anti-stress, and immunomodulatory roles. Its major pharmacological active ingredient is the ginsenoside, and Rg1 is an active ingredient with a higher content.

OBJECTIVE:

To investigate the effect of ginsenoside Rg1 on type II collagen and cyclooxygenase-2 mRNA expression in chondrocytes of an interleukin-1β-induced osteoarthritis model.

METHODS:

Undamaged cartilage from osteoarthritis patients undergoing total knee arthroplasty was harvested and cultured. The effect of ginsenoside Rg1 (0.001, 0.01, 0.1, 1, 10, 100 mg/L) on proliferation rate of passage 2 chondrocytes was analyzed by Cell Counting Kit-8. Then the passage 2 chondrocytes were divided into blank group, control group and experimental group randomly. Dulbecco's modified Eagle's medium was added into the blank group alone. Interleukin-1β at a dose of 10 μg/L was added into the control group to establish an osteoarthritis model. While 10 μg/L interleukin-1β and ginsenoside Rg1 with different concentrations (0.1, 1, 10, 100 mg/L) were added into the experimental group concomitantly. After 24-hour in vitro culture, the expressions of type II collagen and cyclooxygenase-2 gene in human articular chondrocytes were analyzed by reverse transcription-PCR.

RESULTS AND CONCLUSION:

Promotive effect of the certain concentration of ginsenoside Rg1 on chondrocyte proliferation was observed by the result of cell counting kit-8 analysis. However, compared with the blank control group, the lower concentration of ginsenoside Rg1 (0.001, 0.01, 0.1, 1 mg/L) could not stimulate the proliferation rate of chondrocytes significantly (P > 0.05); only the higher concentration of ginsenoside Rg1 (10 and 100 mg/L) could stimulate the proliferation rate of chondrocytes significantly (P < 0.05). From the results of cell counting kit-8 analysis, compared with the blank group, the type II collagen mRNA expression significantly decreased and cyclooxygenase-2 mRNA expression significantly increased in chondrocytes of the control group (P < 0.05). When 10 μg/L interleukin-1β and ginsenoside Rg1 of different concentrations (0.1 and 1 mg/L) were added concomitantly in the experimental group, the type II collagen and cyclooxygenase-2 mRNA expression had no obvious changes compared with the control group (P > 0.05). Meanwhile, when 10 μg/L interleukin-1β and ginsenoside Rg1 of different concentrations (10, 100 mg/L) were added concomitantly in the experimental group, the type II collagen mRNA expression significantly increased and cyclooxygenase-2 mRNA expression significantly decreased compared with the control group (P < 0.05). These findings showed that interleukin-1β induced decreased mRNA expression of type II collagen and increased mRNA expression of cyclooxygenase-2 could be antagonized by somewhat concentration of ginsenoside Rg1.

RESULTS AND CONCLUSION:

Zhang YY, Cheng WD, Chen ZF, Fan WM, Liu F. Effect of ginsenoside Rg1 on type II collagen expression in chondrocytes. Zhongguo Zuzhi Gongcheng Yanjiu. 2013;17(11): 1917-1924.

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