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Abstract

BACKGROUND:

Long-term clinical research has demonstrated that Huangqi Sanxian decoction exerts a certain curative effect on osteoporosis. In addition, experimental studies have also showed that Huangqi Sanxian decoction can improve the biomechanical indexes in experimental animals. The present study aimed to investigate the effect of Huangqi Sanxian decoction on osteoblasts in the cellular level.

OBJECTIVE:

To observe the effects of Huangqi Sanxian decoction on gene regulation of osteoprotegerin and receptor activator of nuclear factor kappa B ligand in osteoblasts cultured in vitro and to investigate the action mechanism underlying the curative effect of Huangqi Sanxian decoction on osteoporosis.

METHODS:

Huangqi Sanxian decoction, Xianlinggubao capsule containing serum and blank serum were prepared to intervening osteoblasts from newborn Sprague-Dawley rats cultured in vitro. Cellular morphology was observed, and western blot analysis and determination of protein concentration were used to observe the effects of drug-containing serum on osteoblast proliferation, gene expression of osteoprotegerin and receptor activator of nuclear factor kappa B ligand in osteoblasts cultured in vitro.

RESULTS AND CONCLUSION:

After drug intervention, osteoblasts were spindle-shaped and trabs-shaped, which were concentrated and confluent with undefined boundary. Huangqi Sanxian decoction could increased the protein expression of osteoprotegerin in osteoblasts (P < 0.01), and also increased the protein expression of receptor activator of nuclear factor kappa B ligand (P < 0.01) to increase ratio of osteoprotegerin/receptor activator of nuclear factor kappa B ligand (P < 0.01). Huangqi Sanxian decoction can achieve the therapeutic effect on osteoporosis through regulating the expression of osteoprotegerin and receptor activator of nuclear factor kappa B ligand.

RESULTS AND CONCLUSION:

Li XM, Lu CJ, Zhou YF, Zhou ZK, Feng X, Zheng LH. Huangqi Sanxian decoction is involved in gene regulation of osteoprotegerin and receptor activator of nuclear factor kappa B ligand in osteoblasts. Zhongguo Zuzhi Gongcheng Yanjiu. 2013;17(11): 1939-1945.

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