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Up to now, the mechanism underlying interferon alpha 2a to improve hepatic fibrosis has not been clarified.


To investigate the effect of interferon alpha 2a on hepatic stellate cells apoptosis in the CCI4-induced hepatic fibrosis rat model.


We established the CCI4-induced hepatic fibrosis models in rats. Fifty healthy female Sprague-Dawley rats were equally and randomly divided into five groups, certainly each group included 10. Five groups were saline control group, hepatic fibrosis model group (model group), 6×104 U/kg interferon alpha 2a intervention group, 12×104 U/kg interferon alpha 2a intervention group and 6×104 U/kg interferon alpha 2a control group. At 8 weeks after modeling, blood and liver tissues were collected to detect the indicators of hepatic fibrosis; the expression of bcl-2 and bax in the liver tissue was analyzed with semi-quantitative reverse transcription-PCR; and immunohistochemical staining was used to mark a-smooth muscle actin in activated hepatic stellate cells.


Pathological morphology of the liver tissue demonstrated that the hepatic fibrosis model was successfully established. The model group had fibrosis significantly around the portal area; in addition, Mans-like fibers and fibrous septa formed. Different interferon alpha 2a intervention groups had fibrosis relief to different extent. a-smooth muscle actin had a great amount of positive expression in the model group, while the positive expression of a-smooth muscle actin was lower in the interferon alpha 2a intervention groups, especially in the 12×104 U/kg interferon alpha 2a intervention group as compared the model group. In addition, there was no expression of a-smooth muscle actin in the 6×104 U/kg interferon alpha 2a control group. Interferon alpha 2a could down-regulate bcl-2 expression and up-regulate bax expression in CCI4-induced hepatic fibrosis models. These findings indicate that the mechanism of interferon alpha 2a blocking CCI4-induced hepatic fibrosis is mainly present by regulating the expression of bcl-2 and bax to induce apoptosis of hepatic stellate cells, and this regulatory role is possibly related to interferon alpha 2a dose.


Liu CY, Zhang W, Liu PP, Ye CG, Yi Z, Sun SL. Interferon alpha-2a and apoptosis of hepatic stellate cells in rats with hepatic fibrosis. Zhongguo Zuzhi Gongcheng Yanjiu. 2013;17(11): 1987-1992.

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