Symbol: 1-Ethy1-3-(3-dimethylaminopropy1)carbodiimide hydrochloride cross-linking xenogeneic porcine acellular tendon for repairing Achilles tendon injury

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Abstract

BACKGROUND:

Heterogeneous tendon is an ideal method to repair tendon defects, but the post-transplantation rejection limits the application of heterogeneous tendons.

OBJECTIVE:

To investigate the effect of transplantation of 1-ethy1-3-(3-dimethylaminopropy1) carbodiimide hydrochloride cross-linking acellular tendon of Banna inbred-lines miniature pig for repairing the rabbit Achilles tendon defects.

METHODS:

Lateral resection of bilateral Achilles tendons from 40 Japanese rabbits was performed, and then the tendons were divided into experimental group and control group. The experimental group was repaired with transplantation of 1-ethy1-3-(3-dimethylaminopropy1) carbodiimide hydrochloride cross-linking acellular treated tendon of Banna mini-pig inbred-lines, and the control group was repaired with autologous tendon graft.

RESULTS AND CONCLUSION:

Histological observation showed that the cells in the new tissue of two groups were mainly the mononuclear fibroblast and fibrocytes, and the cells were oval or circular fibroblasts at 1-4 weeks after operation; new collagen formation could be seen around the cells in the cell aggregation area, and the collagen represented disorderly; focal cord-like mature collagen was distributed in island shape and formed the so-called “collagen island”; at 12 weeks after operation, the cells were increasingly elongated and became spindle-shaped and long strip fibroblasts. Laboratory testing showed that there were no significant differences in white blood cells, C-reactive protein levels, hydroxyproline content and tensile strength between two groups. The results show that 1-ethy1-3-(3-dimethylaminopropy1) carbodiimide hydrochloride cross-linking acellular tendon from Banna mini-pig inbred-lines can repair rabbit Achilles tendon defects successfully with good histocompatibility, less transplant rejection and strong biomechanical performance.

RESULTS AND CONCLUSION:

Jin XH, Gao CG, Cui SJ, Liu GH, Yang JB, Lin YQ. 1-Ethy1-3-(3-dimethylaminopropy1)carbodiimide hydrochloride cross-linking xenogeneic porcine acellular tendon for repairing Achilles tendon injury. Zhongguo Zuzhi Gongcheng Yanjiu. 2013;17(12): 2153-2158.

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