Symbol: Acetylation attenuates polyamidoamine cytotoxicity

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Abstract

BACKGROUND:

Polyamidoamine dendrimer nanomaterials have been widely used in drug carrier research, but there are many electropositive amino groups on the surface of the entire generation polyamidoamine, resulting in certain cytotoxicity.

OBJECTIVE:

To study the influence of acetylation on polyamidoamine cytotoxicity.

METHODS:

(1) 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay: the cell proliferation of 293T cells incubated with acetylated polyamidoamine under 0, 0.125, 0.25, 0.5, 1, 2, 4 μmol/L concentrations was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. (2) Cell morphology: the cell morphology of 293T cells incubated with 4 μmol/L acetylated polyamidoamine was observed by inverted fluorescence microscope. (3) Cell cycle: the cell cycle of 293T cells incubated with acetylatedpolyamidoamine under 0, 5, 10, 15, 20 mg/L concentrations was detected by flow cytometry.

RESULTS AND CONCLUSION:

Polyamidoamine had cytotoxicity to 293T cells. The cell viability at 4 μmol/L concentration after 48 hours incubation was only 52%, and the acetylation could significantly decrease the cytotoxicity of polyamidoamine (P < 0.01). 293T cells incubated with polyamidoamine shrank and had bad stretching, while 293T cells incubated with acetylated polyamidoamine had good stretching. Acetylated polyamidoamine had no significant effect on the cell cycle, but polyamidoamine at 20 mg/L could block the cell cycle at S stage. All the results show that acetylation can decrease the cytotoxicity of polyamidoamine.

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