Vascular endothelial growth factor can participate in tissue repair and promote wound healing.OBJECTIVE:
To observe the infection rate and vascular endothelial growth factor expression during the healing of rat skin wound following treated with human amniotic membrane preserved by the safflower injection.METHODS:
Rats were randomly divided into four groups after skin wound modeling. The wounds were stuck with gauze, amniotic membrane and amniotic membrane saved with safflower injection. Skin tissue samples of rats were taken at 2, 3, 5, 7 days after wound for immunohistochemical staining. Then the expressions of vascular endothelial growth factor and infection rate were detected with auto image analysis system.RESULTS AND CONCLUSION:
The expression of vascular endothelial growth factor was much higher in the rats treated with human amniotic membrane saved with safflower injection than the others at days 2 and 3 after wound (P < 0.05). The infection rate of skin wound in the rats treated with human amnion saved with safflower injection (5%) was less than that of the other groups at day 5 after wound (P < 0.05). Results showed that the human amniotic membrane preserved by safflower injection could not only enhance the expression of vascular endothelial growth factor around the area of injury in the initial stage, but also reduce the infection rate of skin wound, thereby promoting wound healing in rats.