Telomere-associated proteins will directly affect the function of telomeres, adjust the length of telomeric DNA, which are closely related with cell senescence and carcinogenesis.OBJECTIVE:
To find the key regulatory molecules in the cell senescence process through observing the telomere-associated factor expression in normal cell replicative senescence process.METHODS:
Based on established cell replicative senescence model, reverse transcription-PCR and western blot were used to detect the telomere-associated factor expression on the molecular and protein levels, including the telomere-associated factor human telomere binding protein 1, tankyrase 1, telomerase RNA, telomere protection protein 1 and P53 expressions in the human embryonic lung fibroblast replicative senescence.RESULTS AND CONCLUSION:
The results showed that with the cell senescence, transcription of human telomere binding protein 1 did not changed, while the protein expression of human telomere binding protein 1 was increased gradually and then decreased rapidly; mRNA and protein expressions of telomere protection protein 1 did not changed; with the human embryonic lung fibroblast replicative senescence, expression of telomere protection protein 1 was decreased gradually; with cell senescence, telomerase RNA component showed an increasing trend; protein expression of P53 did not changed. Human telomere binding protein 1, telomere protection protein 1 and telomerase RNA play an important role in cell senescence.