Periodontal ligment stem cells are adult stem cells derived from dental tissues, which possess the capability of osteogenic differentiation. They are promising “seed cells” in bone tissue engineering.OBJECTIVE:
To investigate the effects of osteogenic induction medium on the osteogenic differentiation and early apoptosis of periodontal ligment stem cells.METHODS:
Periodontal ligment stem cells were isolated from primary periodontal ligament tissues and seeded on the plate at a density of 1×104/cm2. Osteogenic induction medium containing 1, 10 and 100 nmol/L dexamethasone, β-glycerophosphate and ascorbic acid were used. Alkaline phosphatase activity, mineral deposition staining and real time PCR were performed to assess the osteogenic capacity. AnnexinV/PI staining was used to detected apoptosis.RESULTS AND CONCLUSION:
Dexamethasone effectively promoted osteogenic differentiation of periodontal ligment stem cells, by up-regulating alkaline phosphatase activity, enhancing mineral deposition and increasing osteogenic related gene (osteonectin and type I collagen) expression. Alkaline phosphatase activity and mineralized nodule detection showed that osteogenic effects of dexamethasone were gradient-dependent, and 100 nmol/L was the best concentration. Apoptosis detection results indicated that osteogenic differentiation of dexamethasone for periodontal ligment stem cells could also induce cell apoptosis to some extent.Subject headings:
stem cells; periodontal ligament; dexamethasone; alkaline phosphatase; apoptosisFunding:
the Science and Technology Program of Medical Health of Zhejiang Province, No. 2011KYB003*, 2012KYB056*Funding:
Gu ZY, Zhai Q, Lü QF, Peng CX, Liu FF, Peng H. Dexamethasone induces osteogenic differentiation and apoptosis of periodontal ligament stem cells.