Adipose-derived stem cells are a population of multilineage cells isolated from adipose tissue, which may have a positive effect on the treatment of ischemic diseases.OBJECTIVE:
To explore the therapeutic and angiogenic effects of adipose-derived stem cells via local transplantation on random pattern skin flaps in mice.METHODS:
Human adipose-derived stem cells were isolated, cultured and passaged in vitro. On the back of the SPF mice, random pattern skin flaps were performed. After the operation, the adipose-derived stem cells were injected into the pedicle, central, and distal end of the flaps in the experimental group, while only PBS was injected into the control flaps. Seven days later, the survival rate of flaps was evaluated. Immunofluorescence assay was preformed to observe the distribution of microvessels in the flaps and trace the CM-Dil labeled adipose-derived stem cells, while the level of vascular endothelial growth factor was tested by ELISA and the protein expression of stromal cell-derived factor 1 tested by western blot at day 14 after adipose-derived stem cells transplantation.RESULTS AND CONCLUSION:
Compared with the control group, there was a significant increase in the flap survival rate in the experimental group, and along with the sharply increased number of microvessels, the secretions of vascular endothelial growth factor and stromal cell-derived factor 1 were also obviously raised in the experimental group (P < 0.05). After local transplantation of adipose-derived stem cells into random skin flaps, it could intervene the secretion of vascular endothelial growth factor and stromal cell-derived factor 1 and promote angiogenesis of the skin flap.