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Abstract

BACKGROUND:

Bone marrow mesenchymal stem cells can differentiate into renal tubular epithelial cells. It is speculated that mesenchymal stem cells may have a therapeutic role in chronic renal dysfunction.

OBJECTIVE:

To study the effect of bone marrow mesenchymal stem cells on the aging kidney of rats and its possible mechanism.

METHODS:

Aging models were established in rats. Rat bone marrow mesechymal stem cells cultured in vitro were labeled by 5,6-carboxyfluorescein diacetate, succinimidyl ester, and then injected into the rats via the tail vein. Under a fluorescence microscope, the cell homing was observed. The contents of urea nitrogen and creatinine in serum of rats were tested by automatic biochemical analyzer. The overlaying of blue fluorescent 2-(4-amidinophenyl)-6-indolecarbamidine dihydrochloride and the specific protein of red fluorescent tubular epithelial cells (keratin protein) was detected by indirect immunofluorescence and computer image stacking technique.

RESULTS AND CONCLUSION:

Bone marrow mesenchymal stem cells could arrive the kidney of rats. Compared with the model group, the contents of urea nitrogen and creatinine in serum were decreased in the treated group (P < 0.05). The treated group showed a small amount of double positive cells. These findings indicate that rat bone marrow mesenchymal stem cells may differentiate into renal tubular epithelial cells, which may be one of mechanisms that improve the aging kidney of rats.

RESULTS AND CONCLUSION:

Funding: the Science and Technology Plan Fund of Guizhou Province, No. SY[2010]3075; the Grant from the Health Bureau of Guizhou Province, No. gzwkj2011-1-023

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