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Abstract

BACKGROUND:

Adipose-derived stem cells have similar characteristics of bone marrow mesenchymal stem cells, including adhesion and fibroblast-like clone formation. In addition, adipose-derived stem cells can differentiate into bone, adipose, cartilage.

OBJECTIVE:

To compare the biological characteristics of C57 mouse adipose-derived and bone marrow mesenchymal stem cells.

METHODS:

Mesenchymal stem cells were obtained respectively from the adipose and bone marrow of C57 mice under sterile condition. Adipose-derived and bone marrow mesenchymal stem cells were isolated and purified in vitro to determine cell morphology, suface marker, growth kinetics, differentiation potential and Notch signal related gene.

RESULTS AND CONCLUSION:

Morphology was similar between adipose-derived and bone marrow mesenchymal stem cells under an optical microscope. Adipose-derived and bone marrow mesenchymal stem cells at passage 3 expressed CD29, CD105, Sca-1, but not expressed CD34, CD133. In addition, bone marrow mesenchymal stem cells also expressed CD45. The growth kinetics and cell clone analysis indicated that the proliferation rate of adipose-derived mesenchymal stem cells was significantly faster than that of bone marrow mesenchymal stem cells. Both of adipose-derived and bone marrow mesenchymal stem cells were induced to osteoblasts, adipocytes and chondrocytes, but the adipose-derived mesenchymal stem cells were easier to the osteogenetic differentiation than bone marrow mesenchymal stem cells. Notch signal related gene detection shown that adipose-derived mesenchymal stem cells expressed lower levels of Jagged-1 mRNA, but higher levels of Hes-1 mRNA compared with bone marrow mesenchymal stem cells. These findings suggest that the proliferation rate of adipose-derived mesenchymal stem cells is significantly faster than that of bone marrow mesenchymal stem cells, and the former is apt to the osteogenetic differentiation and may be related with the expressed levels of Hes-1 mRNA.

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