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Abstract

BACKGROUND:

Adipose-derived mesenchymal stem cells are a group of pluripotent stem cells, and under certain conditions can differentiate into neural stem cells in vitro.

OBJECTIVE:

To investigate the proliferative and differentiation ability of different passage mesenchymal stem cell from adipose tissue into neurospheres.

METHODS:

The adipose-derived mesenchymal stem cells from Sprague-Dawley rats were separated and cultured in vitro, and morphology and proliferation rate of cells were observed and compared respectively at passages 3, 6, 10 and 20. The cell surface antigens and cells cycle were identified by flow cytometry. Furthermore, adipose-derived mesenchymal stem cells were induced into neurospheres, and the neurosphere rate was counted.

RESULTS AND CONCLUSION:

Adipose-derived mesenchymal stem cells were mainly in long spindle shape, and cells at different passages had highly proliferative capacity in vitro. Except passage 3, adipose-derived mesenchymal stem cells strongly expressed CD29, CD44, CD73 and lowly expressed CD45 and CD34. The proportion of G 0/G1 phase in cell cycle was 93.4% at passage 3, 92.7% at passage 6, 92.4% at passage 10, 86.0% at passage 20. Adipose-derived mesenchymal stem cells at passages 6 and 10 were easier to differentiate into neurospheres than those at passage 20 (P < 0.05), but cells at passage 3 were difficult to differentiate into neurospheres. Therefore, when using adipose-derived mesenchymal stem cells as seed cells, we should pay attention to choose the appropriate amplification passage in order to obtain the cells with best differentiation potential and cell purity.

Funding:

the National Natural Science Foundation of China, No. 81060106; Xinjiang Graduate Research Innovation Project, No. XJGRI2013087

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