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Abstract

BACKGROUND:

Some scholars believe that anesthesia can improve the quality of cord blood separation and collection, and subarachnoid block is commonly used in cesarean section, but there is no research on the influence of subarachnoid block on differentiation potential of mononuclear cells into neural stem cells and astrocytes.

OBJECTIVE:

To study the influence of subarachnoid block on differentiation potential of mononuclear cells in the cord blood of neonates into neural stem cells and astrocytes.

METHODS:

Mononuclear cells isolated from 20 neonates delivered spontaneously and 20 neonates born by cesarean delivery undergoing subarachnoid block were cultured in vitro, acting as control group and study group. After delivery, cord blood samples were taken to isolate and culture mononuclear cells in vitro. After 3 days of routine culture, the cells were subject to non-induced culture and induced differentiation into neural stem cells. The cultured cells were identified by the cell makers Nestin and glial fibrillary acidic protein with immunohistochemistry identification.

RESULTS AND CONCLUSION:

The Nestin and glial fibrillary acidic protein showed no difference between the two groups after induced differentiation (P > 0.05), indicating subarachnoid block has no impact on the differentiation of mononuclear cells in the cord blood from neonates into neural stem cells and astrocytes.

Funding:

the Scientific Research Project of Shanghai Health Bureau, No. 2009139

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