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Abstract

BACKGROUND:

Human placenta is a stable source for mesenchymal stem cells, which is becoming a cell source in the regenerative medicine that attracts widespread attentions.

OBJECTIVE:

To compare the biological characters of mesenchymal stem cells that separated from different components of human placenta (human chorion and villous trophoblast).

METHODS:

The amniotic membrane of placenta surface was detached surgically. Human chorion and villous trophoblast in the fetal side was cut into pieces. After digestion with PBS containing collagenase II, mononuclear cells were separated and cultured in Dulbecco 's modified Eagle 's medium containing 10% fetal bovine serum in 37 °C and 5% CO2, 95% saturated humidity, after 48 hours the full amount in liquid, dislodge suspension cells. Forty-eight hours later, the medium was changed completed, and non-adherent cells were removed.

METHODS:

When cell fusion reached about 90%, trypsin digestion was employed for cell passage. Biological characters of mesenchymal stem cells separated from different components of human placenta were compared through observation of total number of primary cells, cell morphology, and surface markers expression (CD90, CD73 and CD105).

RESULTS AND CONCLUSION:

The flow cytometric analysis revealed that the cells separated from the human chorion and villous trophoblast were over 90% strongly positive for CD90, CD73, CD105. These two sources of cells showed typical fibroblast morphology, suggesting that the cells have the characteristics of mesenchymal stem cells. Under the same enzyme digestion time, enzyme concentration, and shaking speed, more cells are visible from the chorion, and the subsequent culture is easier to harvest cells.

RESULTS AND CONCLUSION:

Hong Y, Huo SW, Lu Y, Zhang Y. Biological characters of mesenchymal stem cells separated from different components of human placenta. Zhongguo Zuzhi Gongcheng Yanjiu. 2014;18(19):3082-3087.

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