Influence of geniposide on collagen II synthesis of cultured chondrocytesin vitro

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Abstract

BACKGROUND:

Chinese herb Cape Jasmine Fruit can activate the chondrocytes and geniposide is an important component in this herb.

OBJECTIVE:

To investigate the effect of geniposide on collagen II synthesis in rat chondrocytes cultured in vitro.

METHODS:

Rat chondrocytes were separated and cultured in vitro. The chondrocytes were then interfered with 25, 50 and 100 mmol/L geniposide. Normal control group was also set. Collagen II mRNA and protein expression was detected with semi-quantitative RT-PCR and western blot analysis, respectively.

RESULTS AND CONCLUSION:

RT-PCR and western blot analysis results showed that, geniposide at 25, 50 and 100 mmol/L increased the collagen II mRNA and protein expression (P < 0.01). Geniposide can promote the synthesis of collagen II in rat chondrocytes cultured in vitro.

Subject headings: chondrocytes; arthritis; collagen; cellular structure; articular cartilage

Funding: the Natural Science Foundation of Hubei Province in China, No. 2010CDB10701

Tan YL, Bao TZ, Liu Q, Han Y, Wang YL, Liu Y, Zhou NX, Yan F, Zhao LT. Influence of geniposide on collagen II synthesis of cultured chondrocytes in vitro. Zhongguo Zuzhi Gongcheng Yanjiu. 2014;18(20):3149-3152.

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