The endothelial dysfunction is the pathogenesis of arteriosclerotic disease, the quantity and function of endothelial progenitor cells are decreased within the cycle, leading to a poor capacity of neovascularizatio, the efficacy of stem cell transplantation alone is unclear, the combination of cytokines and gene-modified stem cells is the hotspot.OBJECTIVE:
To observe the effect of stromal cell-derived factor-1 on the neovascularization after endothelial progenitor cells transplantation.METHODS:
Unilateral hindlimb ischemia model was established in 20 athymic nude mice, and the mice were randomly divided into four groups: combined group (intravenous endothelial progenitor cells+intramuscular stromal cell-derived factor-1), endothelial progenitor cells group (intravenous injection of endothelial progenitor cells), stromal cell-derived factor-1 group (intramuscular injection of stromal cell-derived factor-1), and blank control group (intramuscular M199). The skin temperature of ischemic hindlimbs and survival of animals after transplantation were observed. The ratio of capillary/skeletal muscle fiber was counted. The expression of CD31 and endothelial nitric oxide synthase were detected.RESULTS AND CONCLUSION:
The fluorescence-labeled endothelial cells were embedded in ischemic hindlimb muscles after cell transplantation. Of the 20 nude mice, two mice died. The rate of ischemic hindlimb reserving was respectively 80%, 75%, 20% and 0 in combined group, endothelial progenitor cells group, stromal cell-derived factor-1 group, and blank control group. The capillary/muscle fiber ratio in combined group and endothelial progenitor cells group was higher than that of blank control group (P < 0.01). The combined group was greater than endothelial progenitor cells group, and endothelial progenitor cells group was greater than stromal cell-derived factor-1 group (P < 0.05). The capillary density in combined group and endothelial progenitor cells group were higher than that in blank control group (P < 0.01), and stromal cell-derived factor-1 group was also more than blank control group (P < 0.05). The combined group was greater than endothelial progenitor cells group, and endothelial progenitor cells group was greater than stromal cell-derived factor-1 group (P < 0.05). The positive rate of endothelial nitric oxide synthase was 73.33% and 53.33% in combined group and endothelial progenitor cells group respectively (P > 0.05). Endothelial progenitor cells can migrate to ischemic tissues, endothelial progenitor cells transplantation can promote neovascularization, and stromal cell-derived factor-1 augments the neovascularization after cell transplantation, in which endothelial nitric oxide synthase is involved.
Subject headings: cell transplantation; cytokines; neovascularization, physiologic; nitric oxide synthase
Wu YB, Wang YQ, Fu WG, Zhu YF, Ge HW. Stromal cell-derived factor-1 and endothelial progenitor cells improve neovascularization. Zhongguo Zuzhi Gongcheng Yanjiu. 2014;18(20):3158-3164.