Effect of basic fibroblast growth factor on human periodontal ligament cells culturedin vitro

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Abstract

BACKGROUND:

Basic fibroblast growth factor is a pluripotent cytokine that can promote the proliferation of mesodermal and neuroectodermal cells.

OBJECTIVE:

To observe the effect of basic fibroblast growth factor in human periodontal ligament cells cultured in vitro.

METHODS:

Human periodontal ligament cells at passage 5 were inoculated into the 96-well plates at the density of 1×108/L, and were randomly divided into four groups. The cells were cultured in α-MEM containing 15% fetal bovine serum and 0, 1, 10, 100 μg/L basic fibroblast growth factor, respectively. At 1, 3, 5, 7 days of the culture, the cell proliferation was determined, and the activity of alkaline phosphates was detected at 1 and 7 days.

RESULTS AND CONCLUSION:

There were significant differences in the proliferation of human periodontal ligament cells among the four groups (F=6.586, P=0.024). As the increase of the basic fibroblast growth factor concentrations, the absorbance value was gradually increased and reached the peak in 100 μg/L basic fibroblast growth factor group (P < 0.05). The alkaline phosphatase activity in basic fibroblast growth factor groups was lower than that of the control group (P=0.000), the higher the concentration was, the lower activity was (P < 0.05). Results show that basic fibroblast growth factor can promote the proliferation of human periodontal ligament cells and inhibit the activity of alkaline phosphatase, and the effect is concentration-dependent.

RESULTS AND CONCLUSION:

Subject headings: periodontal ligament; cells; fibroblast growth factor 2; cell proliferation

RESULTS AND CONCLUSION:

Lin YD, Zhao B, Yin K. Effect of basic fibroblast growth factor on human periodontal ligament cells cultured in vitro. Zhongguo Zuzhi Gongcheng Yanjiu. 2014;18(20):3184-3189.

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