Effect of Breviscapine on the survival and growth of rat cortical neurons culturedin vitro

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Abstract

BACKGROUND:

Nerve growth factor plays an irreplaceable role on the survival, differentiation and maintenance of the central nervous system cells, also promote the growth and development of these cells.

OBJECTIVE:

To explore effect of Breviscapine on the growth of in vitro cultured neurons in cerebral cortex of Sprague-Dawley rats, and preliminarily investigate the action mechanism.

METHODS:

The cerebral cortex of newborn Sprague-Dawley rat embryos was collected, the neurons were primary cultured and randomly divided into three groups: normal control group (no treatment), control group (neurons were cultured with normal saline), Breviscapine group (neurons were cultured with 10 g/L Breviscapine). Furthermore each group was assigned into 24-hour and 48-hour subgroups. Images were captured from the 24-well plates at each time points. RT-PCR was applied to examine the expression of nerve growth factor and TrkA mRNA. MTT was used to detect the neuronal growth at each time point.

RESULTS AND CONCLUSION:

There was no difference between normal control group and control group in the cell counts, cell body area, neurite length and viability (P > 0.05), as the time prolonged, all data were raised in the two groups (P < 0.05). Breviscapine group showed a higher cell count, cell body area, neurite length and viability than normal control group and control group (P < 0.05). No significant difference was found in the expression of nerve growth factor and TrkA mRNA between normal control group and control group (P > 0.05). At each time point, these data in Breviscapine group were increased compared with normal control group and control group (P < 0.05). Breviscapine can promote the survival and growth of brain-derived neurons in Sprague-Dawley rats, and the mechanism might depend on the up-regulating expression of nerve growth factor and its receptor TrkA.

Subject headings: drugs, Chinese herbal; nerve growth factor; receptor, TrkA

Funding: the National Natural Science Foundation of China, No. 81260075, 31260253; Applied Basic Research Jointly by Yunnan Provincial Science and Technology Bureau-Kunming Medical University, No. 2012FB002, 2012FB092; Medical Engineering Technology Research Center for Tumor Transformation of Yunnan Province, No. 2011DH011

Li JY, Wang DY, Yang JW, Zhao N, Li LY, Cheng JR, Ru J, Guo JH, Liu J. Effect of Breviscapine on the survival and growth of rat cortical neurons cultured in vitro. Zhongguo Zuzhi Gongcheng Yanjiu. 2014;18(20):3196-3201.

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