Effect of different concentrations of human amniotic homogenate supernatant on the proliferation of rat Schwann cells

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Abstract

BACKGROUND:

Schwann cells are important cell lines in the process of repairing peripheral nerve injury, and human amniotic homogenate supernatant is shown to secrete a variety of cytokines, which could promote the proliferation of Schwann cells.

OBJECTIVE:

To investigate the effect of different concentrations of human amniotic homogenate supernatant on the proliferation of rat Schwann cell 96.

METHODS:

Schwann cell 96 was cultured with high-glucose DMEM containing 20% fetal bovine serum, and the second generation of Schwann cell 96 was applied for experiments. The cultured cells were divided into five groups according to different volume fractions of human amniotic homogenate supernatant (0%, 10%, 15%, 20%, 25%) in the medium.

RESULTS AND CONCLUSION:

The total protein concentration of human amniotic homogenate supernatant was 675 μg/mL, in which the concentration of epidermal growth factor, basic fibroblast growth factor and vascular endothelial growth factor were respectively (470.625±2.546), (4.121±0.026) and (0.172±0.002) ng/L. At 1-7 days, the cell proliferation rate of the 10% and 15% concentration groups was greater than that in 20% and 25% concentration groups (P < 0.05); 10% and 15% concentrations promoted cell proliferation, while 20% and 25% concentrations inhibited cell proliferation. There were no significant difference in the viability of Schwann cell 96 between the control group and the experimental group (P > 0.05). Low concentrations (10%, 15%) of human amniotic homogenate supernatant promote the proliferation of Schwann cell 96, while high concentrations (20%, 25%) of human amniotic homogenate supernatant inhibit cell proliferation.

Subject headings: amnion; Schwann cells; cell proliferation; cytokines

Funding: Science Research and Technology Development Project of Guangxi Zhuang Autonomous Region, No. 1140003A-39; Natural Science Foundation of Guangxi Zhuang Autonomous Region, No. 0991290, 2011GXNSFB018107; National Natural Science Foundation of China for Youths, No. 81301634; Military Medical Science Research Project During the Twelfth Five-Year Plan, No. CWS11J277

Liu L, Wang L, Tong YL, Mo YL, Lv L, Chen YP, Yang WX, Lv LF, Zhan Q, Zhu FJ, Xin HM, Gong ZY. Effect of different concentrations of human amniotic homogenate supernatant on the proliferation of rat Schwann cells. Zhongguo Zuzhi Gongcheng Yanjiu. 2014;18(20):3218-3222.

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