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Abstract

BACKGROUND:

Studies have shown that the surface hydrophilicity and hydrophobicity (i.e., surface wettability) are important factors affecting bacterial adhesion.

OBJECTIVE:

To observe the bacteriostatic action on Staphylococcus aureus after changing wettability on TiAl6Vi4 plates by surface modification.

METHODS:

TiAl6Vi4 plates were buffered and polished, then divided into three groups, ten plates in each group. T1 group was prepared for TiO2 nanometer film on TiAl6Vi4 surface by electrochemically anodic oxidation method, and self-assembled PETS. T2 group was only prepared for TiO2 nanometer film. T3 group was only self-assembled PETS. All the groups were observed by contact angle measurements and Staphylococcus aureus adhesion on different surfaces was evaluated immersion in Staphylococcus aureus solution for 2 hours in vitro.

RESULTS AND CONCLUSION:

Staphylococcus aureus on the plates with hydrophilic surfaces (T1 group) aggregated reciprocally and overlapped together in the shape of grape cluster while those on the hydrophobic surfaces (T2 group) displayed a tendency of aggregation, however resulting in monolayer but not in grape-cluster like shape. On the contrary, bacteria on the plates with superhydrophobic surfaces (T3 group) distributed dispersedly with no bacteria-clusters or strings. Furthermore, along with the decline of hydrophilia, bacteria counts decreased gradually, exhibiting an increasing trend of separation among bacteria as well. Absorbance value of T3 group remained significantly higher than that of T1 and T2 groups (P < 0.05). It is indicated that titanium superhydrophobic surfaces are able to reduce Staphylococcus aureus adhesion and useful for prevention implant related infection of orthopedics.

Funding:

the Nursery Foundation for Science and Technology Innovation in PLA General Hospital, No. 13KMM11

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