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Abstract

BACKGROUND:

Clinical trials have proved that nano-hydroxyapatite/collagen bone scaffold has good tissue compatibility, suitable porosity and degradation performance, but it is limited by the lack of sustained-release growth factors and induced osteogenic capacity.

OBJECTIVE:

To study the effect of the sustained-release system of polyethylene pyrrolinone modified nano-hydroxyapatite composite scaffold carrying bone morphogenetic protein.

METHODS:

Samples were divided into three groups, and there were two cryogenic vials in each group. In the experimental group, a piece of 5 mm×5 mm×5 mm nano-hydroxyapatite/collagen bone was placed per vial and mixed with polyethylene pyrrolinone at -4 °C overnight, and bone morphogenetic protein solution was then added followed by vacuum pumping and cryopreservation. In the control group 1, a piece of 5 mm×5 mm×5 mm nano-hydroxyapatite/collagen bone was placed per vial and mixed with bone morphogenetic protein solution followed by vacuum pumping and cryopreservation. In the control group 2, a piece of 5 mm×5 mm×5 mm non-decalcified rat cancellous bone was placed per vial and mixed with polyethylene pyrrolinone at -4 °C overnight, and bone morphogenetic protein solution was then added followed by vacuum pumping and cryopreservation. After 14 days, the in vitro release activity of bone morphogenetic protein in the three groups was detected using enzyme-linked immunosorbent assay.

RESULTS AND CONCLUSION:

After 14 days, the absorbance values of bone morphogenetic protein in the two control groups were close to 0, whereas the experimental group showed a higher absorbance value (P < 0.05). It indicates that polyethylene pyrrolinone modified bone morphogenetic protein/nano-hydroxyapatite composite scaffold presents good sustained-release effects.

Subject headings:

biocompatible materials; bone morphogenetic proteins; brace; delayed-action preparations

Funding:

the Social Development Foundation of Zhenjiang City, No. SH2002019

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