Changes in apoptosis-related genes in bone marrow mesenchymal stem cells after cocultured with hepatic stellate cells

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Abstract

BACKGROUND:

Previous studies have confirmed that bone marrow mesenchymal stem cells in vitro can promote hepatic stellate cell apoptosis and inhibit its activity, in which the mechanism of action remains unknown.

OBJECTIVE:

To screen out apoptosis-related genes during hepatic stellate cell apoptosis regulated by bone marrow mesenchymal stem cells using gene chip technology.

METHODS:

Purified human bone marrow mesenchymal stem cells were seeded in 6-well Transwell plate and cocultured with hepatic stellate cells. Cultured human bone marrow mesenchymal stem cells alone served as control group, and cultured for 72 hours. The alterations in apoptosis-related genes were analyzed between culture alone group and coculture group using gene chip technology. The genes strongly associated with regulation of hepatic stellate cells were selected.

RESULTS AND CONCLUSION:

By the functional classification of second-generation SABiosciences Gene chips, apoptotic gene screening found that after coculture, significantly upregulated genes in bone marrow mesenchymal stem cells contained: AKT1, PIK3R2, DAPK1, DHCR24, NOTCH2 and BDNF. Combined with previous findings, we hypothesized that NOTCH may play a key role in the regulation of hepatic stellate cells by bone marrow mesenchymal stem cells.

Subject headings:

bone marrow; mesenchymal stem cells; hepatic stellate cells; apoptosis; microchip analytical procedures

Funding:

the Doctoral Fund of Ministry of Education of China, No. 20110171120089, 20110171110070

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