Changes in memory function of rats with brain injury after fingolimod administration combined with bone marrow mesenchymal stem cell transplantation

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Abstract

BACKGROUND:

The repair effects of bone marrow mesenchymal stem cell transplantation on brain injury were not ideal. Combined therapy with medicine and biological engineering materials is needed.

OBJECTIVE:

To investigate the effects of bone marrow mesenchymal stem cell transplantation and fingolimod immunosuppressants on memory function recovery in rats with brain injury.

METHODS:

A total of 60 healthy Sprague-Dawley rats were subjected to hydraulic shock with peak value of 253.312 5-303.975 kPa with a hydraulic head injury instrument so as to induce a model of severe hydraulic head injury. They were randomly divided into brain injury group, bone marrow mesenchymal stem cell transplantation group and fingolimod + bone marrow mesenchymal stem cell transplantation group. The Morris water maze test was tested at 21-28 days after PKH-26-labeled bone marrow mesenchymal stem cell transplantation. The PKH-26 immunofluorescence and hematoxylin-eosin staining were conducted in brain tissues at 4 weeks after brain injury.

RESULTS AND CONCLUSION:

At 4 weeks after transplantation, the average escape latency was gradually decreased in each group. The average escape latency was shorter in the fingolimod + bone marrow mesenchymal stem cell transplantation group than in the bone marrow mesenchymal stem cell transplantation group (P < 0.05), and significantly shorter than in the brain injury group (P < 0.01). The number of times of crossing the platform and the percentage of swimming distance to total distance were higher in the fingolimod + bone marrow mesenchymal stem cell transplantation group than in the brain injury group and bone marrow mesenchymal stem cell transplantation group (P < 0.05). The number of PKH-26-positive cells was significantly higher in the fingolimod + bone marrow mesenchymal stem cell transplantation group than in the brain injury group and bone marrow mesenchymal stem cell transplantation group (P < 0.05). Results confirmed that bone marrow mesenchymal stem cell transplantation could apparently improve memory function of rats with severe brain injury. The combined application of fingolimod immunosuppressants has synergistic effects.

Subject headings:

bone marrow; mesenchymal stem cells; brain injuries; immunosuppressive agents

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