Optimal concentration of modified platelet-rich plasma to promote the proliferation of dental pulp stem cells from deciduous teeth

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Abstract

BACKGROUND: Previous experiments have shown that modified platelet-rich plasma activated by liquid nitrogen freezing and thawing can promote the proliferation of human bone marrow mesenchymal stem cells and dental pulp stem cells in a concentration-dependent manner.

OBJECTIVE: To investigate the effect of modified platelet-rich plasma at different concentrations on the proliferation of dental pulp stem cells from human exfoliated deciduous teeth.

METHODS: Platelets were selected and harvested by automatic blood cell analyzer, and then activated by liquid nitrogen freezing and thawing. α-MEM served as basal medium. Different concentrations of modified platelet-rich plasma (2%, 5%, 10%, 20%) or 10% fetal bovine serum were added, respectively. The difference in cell proliferation was observed.

RESULTS AND CONCLUSION: Modified platelet-rich plasma at different concentrations could promote the proliferation of dental pulp stem cells from deciduous teeth. The effects of 2% modified platelet-rich plasma and 10% fetal bovine serum on promoting the proliferation of dental pulp stem cells from deciduous teeth were similar. These results indicated that 2% modified platelet-rich plasma could promote the proliferation of dental pulp stem cells from deciduous teeth, and substitute for fetal bovine serum in the amplification of dental pulp stem cells from deciduous teeth in vitro.

Funding: the Guangdong Provincial Science and Technology Project, No. 2011B031800201

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