Human cytomegalovirus detection in hematopoietic stem cell transplantation: value of fluorescence quantitative PCR in the early diagnosis

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To improve the survival rate of transplanted hematopoietic stem cells, dynamic monitoring of plasma content of human cytomegalovirus (HCMV) and rapid screening of early active HCMV infection in hematopoietic stem cell tranplantation recipients, thus to guide the clinical medication, is preferred.


To evaluate the usefulness of fluorescence quantitative PCR assay for early detection of HCMV activation after hematopoietic stem cell transplantation.


Real-time fluorescence quantitative PCR assay was applied for HCMV monitoring in 656 blood samples from 41 hematopoietic stem cell tranplantation recipients and 60 control blood samples.


In 656 blood samples, 96 samples were positive, and the HCMV DNA copies ranged from 5.0×102 to 1.0×107 IU/mL. Timely initiation of therapy resulted in the rapid clearance of DNA-viraemia but it recurrenced in short time by drug-resistent. Two cases from 12 postive recipients died from HCMV infection. The quantitative detection of HCMV DNA by real-time PCR is a rapid method for monitoring HCMV infection and the early diagnosis in patients after hematopoietic stem cell transplantation.

Subject headings:

hematopoietic stem cell transplantation; cytomegalovirus; cytomegalovirus infections; polymerase chain reaction

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