Apoptosis of human embryonic cerebral cortex neurons transfected with human telomerase reverse transcriptase

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Abstract

BACKGROUND:

Human telomerase reverse transcriptase recombinant adenovirus transfection could promote the survival and inhibit the apoptosis of primary cultured human embryonic cerebral cortex neurons.

OBJECTIVE:

To observe the effects of human telomerase reverse transcriptase on the apoptosis of human embryonic cerebral cortex neurons induced by amyloid β-protein invention.

METHODS:

Primary cultured cerebral cortex neurons of human embryo were divided into three groups, namely control group, amyloid β-protein (25-35) group, and human telomerase reverse transcriptase group. Except the control group, the cells were cultured for 144 hours and intervened with 5 μmol/L amyloid β-protein (25-35) for 24 hours. Furthermore, the cells in human telomerase reverse transcriptase group were transfected with human telomerase reverse transcriptase group at 72 hours after intervention of 5 μmol/L amyloid β-protein (25-35).

RESULTS AND CONCLUSION:

After human embryonic cerebral cortex neurons were cultured for 7 days, the apoptotic cells were found in three groups. The apoptosis of neurons was obviously increased after intervention of amyloid β-protein (25-35). Human telomerase reverse transcriptase transfection could prevent the apoptosis of human embryonic cerebral cortex neurons induced by amyloid β-protein (25-35).

Subject headings:

cerebral cortex; apoptosis; telomerase; telomerase-binding proteins; transfection

Funding:

Science and Technology Innovation Foundation of Guangdong Provincial Education Bureau, No. 2012KJCX0089

Funding:

Wu LZ, Li SB, Cheng GW, Wang HQ, Kong LP. Apoptosis of human embryonic cerebral cortex neurons transfected with human telomerase reverse transcriptase. Zhongguo Zuzhi Gongcheng Yanjiu. 2014;18(29):4653-4657.

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