Effect of glutaraldehyde cross-linking on the properties of chitosan/hydroxyapatite-gentamicin delayed materials

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Abstract

BACKGROUND:

Cross-linking is a common method for the modification of bone tissue engineering materials, but there are few studies about the effect of cross-linking on properties of drug-loaded artificial bone.

OBJECTIVE:

To study the effect of glutaraldehyde cross-linking on mechanical strength, degradation rate and in vitro release behavior of chitosan/hydroxyapatite-gentamicin delayed materials.

METHODS:

The chitosan/hydroxyapatite-gentamicin drug-loaded artificial bone and the cross-linked chitosan/hydroxyapatite-gentamicin drug-loaded artificial bone were prepared at 10%, 20% and 30% chitosan mass ratio. The mechanical strength, absorption rate, degradation rate and in vitro release behavior of materials in each group were determined.

RESULTS AND CONCLUSION:

The compressive strength of 10%, 20%, 30% chitosan/hydroxyapatite-gentamicin drug-loaded artificial bone was (10.16±1.17) MPa, (28.4±0.64) MPa and (23.28±1.30) MPa, respectively. After cross-linking, the strength was increased to (36.3±1.20) MPa, (51.6±2.08) MPa and (36.9±3.22) MPa, respectively. The absorption rate and degeneration rate were reduced by cross-linking. In the first day of drug release in vitro, the rate of gentamicin released from non-cross-linked 30% chitosan/hydroxyapatite-gentamicin drug-loaded artificial bone was 42.2%, while the rate was decreased to 33.6% after cross-linking. At the following 9 days, the total release of the cross-linked material was lower than that of non-cross-linked material. Glutaraldehyde cross-linking could improve the stability, reduce the degradation rate, and significantly ameliorate the release of artificial bone.

Subject headings:

hydroxyapatites; chitosan; mechanics; biodegradation, environmental

Funding:

the National Natural Science Foundation of China, No. 31300804, 31301480

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