Effect of Bio-Gide collagen membranes on the proliferation and osteogenetic differentiation of rabbit bone marrow mesenchymal stem cells

    loading  Checking for direct PDF access through Ovid

Abstract

BACKGROUND:

Bio-Gide collagen membranes show a good biocompatibility with stem cells. But the research on the osteogenetic differentiation of bone marrow mesenchymal stem cells cultured on the Bio-Gide collagen membranes is rarely reported.

OBJECTIVE:

To observe the effect of Bio-Gide collagen membranes on the proliferation and the osteogenetic differentiation of bone marrow mesenchymal stem cells.

METHODS:

Bone marrow mesenchymal stem cells from rabbits were isolated and cultured by using the whole bone marrow adherence method in vitro. Passage 3 bone marrow mesenchymal stem cells were selected and seeded on the Bio-Gide collagen membrane pretreated petri dish (experimental group) and simple petri dish (control group). The proliferation of bone marrow mesenchymal stem cells was detected by Cell Counting Kit-8 at 1, 4, 7, 14 days. The supernatant of the cells cultured in osteogenic differentiation medium were collected to detect the activity of alkaline phosphatase at 1, 4, 7, 14 days.

RESULTS AND CONCLUSION:

The number of bone marrow mesenchymal stem cells in the two groups was increased with the increasing time, and the control group had more cells than the experimental group at 7 days (P < 0.05). There was no significant difference between the two groups at other time points. The alkaline phosphatase activity was increased with the increasing culture time, and the experimental group had a higher activity than the control group at 14 days (P < 0.05). There was no significant difference between the two groups at other time points. Experimental findings indicate that Bio-Gide collagen membranes can promote the proliferation and the osteogenetic differentiation of bone marrow mesenchymal stem cells.

Subject headings:

stem cells; collagen; cell differentiation; bone regeneration

Funding:

a grant from General Hospital of the Chinese People's Armed Police Force, No. WZ2012008

Related Topics

    loading  Loading Related Articles