In oral warm and moisture circumstance, alloy which contains Be is easily to be eroded to release Be2+. But there is still no research focusing on beryllium influence on genotype of Porphyromonas gingivalis fimbriae gene cluster.OBJECTIVE:
To investigate Be2+ effect on genotype of Porphyromonas gingivalis fimbriae gene cluster.METHODS:
The revived Porphyromonas gingivalis was resuscitated for 48 hours in the anaerobic culture medium with different concentration of Be2+ (10×10-6, 5×10-6, 1.25×10-6). Through PCR amplification and sequencing, we investigated the effects of Be2+ on genotypes of Porphyromonas gingivalis fimbriae gene cluster.RESULTS AND CONCLUSION:
(1) When Be2+ concentration was 5×10-6, we found the peak of 217 and 257 sites on DNA sequence expressing G/A overlap peak, different from G single peak of the other groups, suggesting the suspicious bases changes, part of the single base G mutated into A. (2) On all concentrations, we found a base group composed of seven A bases was inserted into the 101 site of DNA sequence. Up to now, there is no direct contacts of the mutations occurring to Be2+ concentration. Changes of gene may lead to the shifting of the reading frame, the abnormal synthesis of proteins, the change of Porphyromonas gingivalis fimA gene toxicity, and lastly the unbalance of the micro-ecological environment.Subject headings:
beryllium; Porphyromonas gingivalis; fimbriae, bacterial; pathogenicityFunding:
the Grant from Sichuan Science and Technology Bureau, No. 2008SG0023Funding:
Huang R, Li QY, Lin H, Guo W, Gao N. Influence of beryllium ions on genotype of Porphyromonas gingivalis fimbriae gene cluster. Zhongguo Zuzhi Gongcheng Yanjiu. 2014;18(30):4816-4822.