Mechanical and biological properties of porous calcium phosphate cement/fibrin glue for bone regenerationin vitro

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Abstract

BACKGROUND:

Fibrin glue introduced into calcium phosphate cement has not been confirmed whether this way could overcome the compressive limits and the low degradation of calcium phosphate cement and to modify the biological properties of calcium phosphate cement.

OBJECTIVE:

To explore the mechanical and biological properties of calcium phosphate cement/fibrin glue at different powder/liquid ratio for bone regeneration in vitro.

METHODS:

Calcium phosphate cement and fibrin glue were mixed at ratios of 1:1, 3:1, 5:1 (mL/g), and the pure calcium phosphate cement served as controls. Setting time, scanning electron microscope and the biomechanical test were used to analyze the composite scaffold structure, physical performance and the mechanical properties. Passage 3 osteoblasts were respectively inoculated on the material surface of the four groups, and pure cells served as blank controls. Cell adhesion, proliferation and alkaline phosphatase activity were observed.

RESULTS AND CONCLUSION:

The initial and final setting time of calcium phosphate cement/fibrin glue at 1:1 and 3:1 (mL/g) was higher than that in the control group (P < 0.05), while the initial and final setting time of calcium phosphate cement/fibrin glue at 5:1 (mL/g) was lower than that of the control group (P < 0.05). Scanning electron microscope showed smoother and denser surface of composite scaffolds compared with the pure calcium phosphate cement. The aperture of the composite scaffolds was decreased with the increasing concentration of fibrin glue. The compressive strength of composite scaffolds at 3:1 and 5:1 was higher than that of the control group (P < 0.05), while the modulus of the composite scaffolds at 1:1, 3:1, 5:1 was higher than that of the control group (P < 0.05). Cell adhesion, proliferation and alkaline phosphatase activity showed no difference among the three composite scaffold and control groups, but all higher than the blank control group (P < 0.05). These findings indicate that fibrin glue introduced into calcium phosphate cement can overcome the low-strength limits of calcium phosphate cement, and maintain the good biological properties of calcium phosphate cement for bone regeneration.

Subject headings:

calcium phosphates; osteoblasts; fibrin tissue adhesive

Funding:

the National Natural Science Foundation of China, No. 81000790; Scientific New Star Plan of Beijing City, No. 2010-77

Funding:

Dong JJ, Bi L, Li N, Guo Y, Xu Q, Lei W, Cui G. Mechanical and biological properties of porous calcium phosphate cement/fibrin glue for bone regeneration in vitro. Zhongguo Zuzhi Gongcheng Yanjiu. 2014;18(30):4831-4837.

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