Effect of type I or type II collagen on biological characteristics of human chondrocytes

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Abstract

BACKGROUND:

Experiments have shown that the collagen substrate has the capability of stimulating cartilage generation, but the stimulating role of different types of collagen substrates remains controversial.

OBJECTIVE:

To investigate the effect of type I and type II collagen on the biological characteristics of human chondrocytes cultured in vitro.

METHODS:

Human chondrocytes at passage were cultured onto the ordinary culture plates (ordinary plate), type I collagen-coated culture plates (type I plate), and type II collagen-coated culture plates (type II plate). Cell growth curves were determined by MTT method after cells were cultured for 10 days. By ELISA, PCR, and 1,9-dimethyl methyleneblue technology, type I and type II collagen and glycosaminoglycan contents were quantitatively detected in cartilage cells 28 days after culture.

RESULTS AND CONCLUSION:

The number of cartilage cells was the highest in type II plate, which was twice of that in type I plate and five times of that in ordinary plate. Cartilage cells in type II plate secreted the least amount of type I collagen, which showed significant differences compared with the ordinary plate (P < 0.01) and had no statistically significant difference with type I plate (P > 0.01). Cartilage cells in type II plate secreted the most amount of type II collagen and glycosaminoglycan, showing significant differences compared with the other two plates (P < 0.01). The cartilage cells cultured in collagen plates are better than that cultured in ordinary culture plate, type II collagen culture plate is better than type I collagen culture plate in maintaining cell shape, extending the dedifferentiation pattern, and promoting cell differentiation.

Subject headings:

collagen type I; collagen type II; chondrocytes

Funding:

a grant by Sichuan Provincial Health Ministry

Funding:

Jiang P, Wei P, Zhao MC, Chen Q, Wang Z. Effect of type I or type II collagen on biological characteristics of human chondrocytes. Zhongguo Zuzhi Gongcheng Yanjiu. 2014;18(30):4845-4850.

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