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Selenium (Se)-enriched yeast is a common form of Se for supplementation purpose. In this paper Se retention and metabolism in body, content in natural food and supplements have been introduced. Then recent investigations on the beneficial health effects of Se-enriched yeast addition to foods and impact of process variables in its production as well as, product characteristic, Se species, intended dietary use for Se-enriched yeast, bioavailability and stability have been reviewed. Finally safety of the yeast source in Se-enriched yeast, probable toxicity including acute and chronic toxicity have been discussed. Application of Se-enriched yeast, complying with the general product characteristics outlined in paper when used in food supplements is safe at the proposed intake levels, which upper bound total estimated daily intake for adults was calculated to be 150 g as Se.