To measure serum levels of apoptosis signaling soluble molecules FAS-ligand (sFasL) and FAS-receptor (sAPO-1/Fas) in young and old patients survived myocardial infarction (MI).Methods
We examined 28 young (age 41.5 ± 5.4 yrs.) and 55 old (62.1 ± 8.4 yrs.) pts. Survived MI and 26 quite healthy people with age 41.8 ± 9.7 yrs., p=0.000. Sera samples of both pts. and healthy people underwent measurement of sFasL and sFas levels (ELISA), the concentrations in pts. being measured at 2 weeks' time point after MI.Results
HDL-cholesterol level was lower in young than in old pts. (0.86 ± 0.28 mmol/l vs. 1.00 ± 0.29 mmol/l; p=0.049). Levels of sFasL were 58.38 ± 25.79 pg/ml in quite healthy people, 80.18 ± 40.54 pg/ml in young and 67.79 ± 26.21 pg/ml in old pts. (F=3.511, p=0.033). Post-hoc test revealed significant differences between healthy people and young pts. (z= -2.322, p=0.017) as well as healthy people and old pts. (z= -2.145, p=0.032) though the sFasL concentrations in young and old aged groups didn't differ significantly (z= -1.330; p=0.184). There were no differences in sFas concentrations between groups (p=0.204). In both pts. groups arterial hypertension (AH) predicted higher sFasL levels: sFasL 75.05 ± 33.53 vs. 57.40 ± 21.02 pg/ml (z = -2.484, p=0.013). We did not find differences in types of reperfusion therapy between young and old pts. (χ2=3.454; p=0.327). Type of reperfusion (β=0.420, p < 0.001) and number of arteries with stenosis more than 70% (β=0.279, p=0.016) had a significant effect on sFasL level (R2=0.207, F=8.496, df Residual=65, p=0.001). Reperfusion methods in MI had effects on sFasL concentrations (F=4.465; p=0.006): 66.07 ± 21.37 without reperfusion applied, 64.22 ± 20.00 after primary PCI (pPCI), 62.38 ± 11.80 after thrombolysis only (TL) and 92.65 ± 32.24 pg/ml after combination of TL with PCI. Post hoc test found differences between TL + PCI and pPCI (p=0.008) as well as TL + PCI and reperfusion missing (p=0.032). Although reperfusion did not influence to sFas levels at 2 weeks' time point after MI (F=0.213; p=0.887).Conclusions
Thus, we revealed an increase of apoptosis signaling molecule sFasL serum levels in pts. with MI in comparison with quite healthy people. AH as well as severity of coronary lesion predicted higher sFasL concentrations after MI in both young and old aged groups. Combined reperfusion therapy (TL + PCI) had the strongest short term effect on apoptosis process and was responsible for the highest serum level of sFasL. There were no significant impacts of all the factors listed on serum sApo-1/Fas levels that may suppose sFasL to be more sensitive programmed cell death marker after MI.