P344Increasing of ceruloplasmin ferroksidase activity of revealed in patients with arterial hypertension working under conditions of ultrahigh range radiation exposure

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Abstract

Transferrin level (TF) plays an important role in protecting cells from oxidative damage by radical oxygen species, low levels of ceruloplasmin (CP) is associated with arterial hypertension (AH).

The study purpose was to define the level of CP and TF of patients with AH, working under conditions of microwave electromagnetic radiation (UHF EMR) impact.

Materials and methods

48 men (average age 37,6 ± 6, 4 years) with AH, who worked for 4 through 20 years (average duration15,00 ± 6,4 years) under conditions of UHF EMR impact were tested (group 1). 18 patients were diagnosed with AH I, 30 — with a stage II. UHF EMR dose limit was 4320-31065 kW (average: 17151, 7 ± 7102,4 kW). The control group consisted of male patients with AH, appropriate age, not long-term exposure of UHF EMR (group 2, n = 35). 23 patients with stage I of AH and 12 patients with the stage II were revealed in the 2 group. Both groups' patients were treated with antihypertensive therapy, according to the recommendations of European Society of Cardiology 2007, in the revision of 2009.

Materials and methods

All patients were checked to define the level of CP and TF by the electron paramagnetic resonance EPR computerized spectrometer RE-1307 in the mode of low-temperature stabilization of blood samples. The CP/TF coefficient was calculated.

Results

The level of CP among the patients of the 1 group was higher than the test indicator in the control group (average 1,41 ± 0,69 RU against 0,76 ± 0,06 RU; P < 0,05), whereas the TF level of the 1 group patients was much lower than the control group (0,22 ± 0,02 RU against 0,67 ± 0,03 RU; P < 0,05).

Results

Analysis of the EPR spectrs (the signal from g = 4,25) showed that 38 patients (79%) of the 1 group had a form disturbance of the signals in EPR HF spectr: additional components or form, which corresponded to crystal structure, which indicates testifies about a structural disturbance of the enzyme .

Results

CP level in blood was significantly correlated with the terms of the patients work in condition of UHF EMR (r=0,35; P < 0,05). A negative correlation between the level of HF and UHF EMR exposure period (r=-0,69; P < 0,005) was revealed.

Conclusion

Those patients who worked in condition of UHF EMR were diagnosed with increasing of the CP and reducing TF activity, which reflects the increase in CP ferroksidase activity. The correlation of CP from the period of UHF EMR influence was revealed. The results can be used as a marker of oxidative stress during the damaging influence of UHF EMR on human's body.

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