P447Amelioration of myocardial ischaemia /reperfusion injury by DITPA via modulation of apoptosis and inflammation

    loading  Checking for direct PDF access through Ovid

Abstract

Background: The importance of apoptosis in cell death following ischemia and reperfusion has been demonstrated in vivo rodent models,. Thyroid hormone can have positive effects on the cardiovascular system but its therapeutic potential is limited secondary to its adverse effects. DITPA has been shown to reduce pathologic remodeling and improve cardiac output following myocardial infarction.

Aim of the study: This study was undertaken to assess the possible protective potential of 3, 5-Diiodothyropropionic Acid (DITPA) in regional myocardial ischemia reperfusion injury and apoptosis.

Material and method: Rats were subjected to surgical induction of ischemia and reperfusion by ligated the left interior descending artery (LAD) for 30minutes and 3hr reperfusion and received DITPA or control vehicle, at end of experiment animals sacrificed and blood samples taken to measure cardiac troponin I and cardiac section was taken to make homogenate to measure TNF-α, IL-1β and IL-6.Also cardiac section was taken to make histopathological study and other section to measure apoptosis by ssDNA apoptotic kit.

Result: Compared with sham group, the levels of cardiac TNF-α, IL-1β, IL-6,ssDNA and plasma level of cardiac troponin I (cTnI) increased in control group (p<0.05). Histological, all rats in control group showed significant (p<0.05) cardiac injury. DITPA significantly counteract the increase in the levels of cardiac TNF-α, IL-1β, IL-6,ssDNA and plasma level of (cTnI) in control and control vehicle (p<0.05). Morphologic analysis showed that DITPA markedly improved (p<0.05) the severity of cardiac injury. Conclusions DITPA may ameliorate regional myocardial ischemia reperfusion injury and apoptosis during ischemia via interfering with inflammatory pathway.

Related Topics

    loading  Loading Related Articles