P651Cardioprotective potential of seabuckthorn pulp oil against ischemia reperfusion induced myocardial injury in rats through upregulation of Akt/eNOS pathway

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Abstract

Purpose: Seabuckthorn pulp oil obtained from the fruits of Seabuckthorn (Hippophae rhamnoides L.) has been used since ancient times for its medicinal and nutritional properties. So the present study was done to investigate the cardioprotective effect of Seabuckthorn (SBT) pulp oil in Ischemia-Reperfusion (IR) induced model of myocardial infarction in rats and to evaluate whether this cardioprotective effect involves activation of Akt/eNOS signaling pathway.

Methods: Male albino Wistar rats were divided into three groups: 1-Sham; 2-IR control; 3-SBT pulp oil (20 ml/kg/day; p.o.). SBT pulp oil or vehicle was administered for 30 days. On 31st day, animals underwent left anterior descending coronary artery ligation for 45 min and reperfusion for 60 min.

Results: As compared to sham group, IR injury showed cardiac dysfunction as demonstrated by lowering of mean arterial blood pressure, decrease in maximum positive and negative rate of left ventricular pressure (±LVdP/dtmax) and an increase in left ventricular end-diastolic pressure (LVEDP). Furthermore, there was marked rise in lipid peroxidation, increase in cardiac injury enzyme activities in serum, depletion of antioxidant status along with increase in serum TNF-α and decrease in NO levels. Histopathological study revealed focal necrosis, edema and infiltration of inflammatory cells. Pretreatment with SBT pulp oil (20 ml/kg/day; p.o.) significantly prevented the cardiac necroenzyme release, decreased inflammation, improved cardiac function, restored myocardial oxidant-antioxidant status and decreased the TNF-α level in the serum. Furthermore, it downregulated the IKKβ/NF-κB expression and increased the Akt-eNOS phosphorylation in IR challenged myocardium.

Conclusion: Thus we conclude that SBT pulp oil shields the myocardium against IR injury via modulation of Akt-eNOS and IKKβ/NF-κB expression.

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