Peripheral Gamma Delta T cells secrete inflammatory cytokines in women with idiopathic recurrent pregnancy loss

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Abstract

Background

Gamma delta (γδ) T cells are known to link innate and adaptive immunity. Decidual γδ T cells are known to provide immunotolerance by producing IL-10 and TGF-β. In recurrent pregnancy loss (RPL) females, the role of peripheral γδ T cells remain unstudied.

Objective

To investigate the different phenotypes of γδ T cells in the peripheral blood of women with idiopathic RPL and their possible involvement in RPL condition.

Methods

A total of 120 women were recruited for the study. Peripheral blood lymphocytes were isolated and they were stained with appropriate antibodies to determine the phenotype of γδ T cells and major cytokines produced by them in the blood using flow cytometry.

Results

We observed a significant decrease in the proportion of CD3+CD4−CD8−γδ T cells (p < 0.001) and increase in the percentage of IFN-γ (p < 0.05) and IL-17 (p < 0.001) producing γδ T cells in RPL pregnant as compared to normal pregnant females.

Conclusion

Increase in IFN-γ and IL-17-producing CD3+ CD4−CD8− γδ T cells is associated with creating inflammatory cytokine milieu, thereby, may contribute towards pregnancy loss in RPL females.

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