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Dear Sir,I read the article entitled ‘Fracture resistance and histological findings of immature teeth treated with mineral trioxide aggregate’ (1) published in Dental traumatology journal. I appreciate the great work of the writers; however, this article has a factual error. In this article, the reinforcing effect of the dentin has been explained by increasing expression of TIMP-2 over one year period. As a fact, expression of the genes needs a live system that is the cells must be alive. MTA should trigger the DNA to produce m-RNA and the resultant production of the protein (here: TIMP-2) by ribosomes. All these processes need a live system.

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