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To evaluate the efficacy of oral rehydration salt-liquid as a suitable medium for maintaining the periodontal ligament cell viability over different time periods and to compare its efficacy with that of two other storage media, Hanks' balanced salt solu1tion and milk.A total of 130 sound- and caries-free premolars extracted atraumatically for orthodontic reasons were selected. Of these teeth, 120 premolars were randomly divided into three experimental groups comprising 40 teeth each, for immersion in three different experimental storage media. Each tooth was subjected to 30 or 60 min of extra oral dry time. Each experimental group was further subdivided into two groups comprising 10 teeth each, based on the immersion time of 45 and 90 min, respectively. Of the remaining 10 premolars, five teeth each formed positive and negative controls. All teeth were subjected to collagenase II and dispase assay. Trypan blue dye exclusion test was used to determine the viability of the periodontal ligament cells. The number of viable cells was counted using Neubauer's chamber. Data obtained were subjected to statistical analysis using one-way ANOVA and post hoc Tukey's tests.There was no statistically significant difference between Hanks' balanced salt solution and Oral Rehydration Solution-Liquid.Oral Rehydration Solution-Liquid as a storage medium was found to be as efficient as Hanks balanced salt solution to maintain the viability of periodontal ligament cells, and it was found to be better than milk.