Transcription of the tyrosinase gene family in an Atlantic salmon leukocyte cell line (SHK-1) is influenced by temperature, but not by virus infection or bacterin stimulation

    loading  Checking for direct PDF access through Ovid


The present study was performed to address putative links between the immune and pigmentary systems. A pigment-producing leukocyte-like cell-line (SHK-1 cells) of Atlantic salmon (Salmo salar L.) was exposed to different temperatures, poly I:C, bacterin or infected with virus (infectious pancreatic necrosis virus or infectious salmon anaemia virus). The effect of this stimulation regarding the transcription-pattern of the tyrosinase gene family (melanin genes) and the immune-related genes MHC class II and IFN-1 was analysed using real-time RT-qPCR. At 10 °C cultivation, tyrosinase and dopachrome tautomerase remained unregulated. At 15 °C, a moderate up-regulation was induced, while at 20 °C, these genes were up-regulated in an exponential manner over time. Temperature did not affect the transcription of the immune-related genes. Virus infections, poly I:C or bacterin had no influence on the transcription of the melanogenesis-related genes, but triggered the immune-related genes. Our findings revealed no connections between the pigmentary and immune systems, but demonstrated a hereto undiscovered temperature-effect on the tyrosinase gene family.

Related Topics

    loading  Loading Related Articles