Characterization of a teleost membrane-associated protein that is involved in the regulation of complement activation and bacterial infection


    loading  Checking for direct PDF access through Ovid

Abstract

In mammals, membrane-associated complement regulatory proteins (MCRP) can protect host cells from the damaging of the activated complement. In teleost, few studies on the function of MCRP have been documented. In the present report, we identified a MCRP (named CsMCRP) from the teleost fish tongue sole Cynoglossus semilaevis and examined its immune function. CsMCRP shares moderate sequence identities with fish DAF-like molecules. CsMCRP was predicted to be a transmembrane protein with three short consensus repeats located in the extracellular region. CsMCRP expression occurred in nine different tissues, especially blood, and in peripheral blood leukocytes (PBL). Recombinant CsMCRP inhibited complement activation and interacted with bacterial pathogen, the latter in a highly selective manner. Antibody blocking the CsMCRP on PBL significantly inhibited bacterial infection of PBL. These results indicate that teleost CsMCRP is both a regulator of complement activation and a cellular receptor involved in bacterial invasion.HighlightsCsMCRP is a transmembrane protein of tongue sole with three SCR modules.CsMCRP expression occurred in multi-tissues and peripheral blood leukocytes (PBL).Recombinant CsMCRP (rCsMCRP) inhibited complement activation.rCsMCRP bound bacteria in a highly selective manner.Antibody blocking the CsMCRP on PBL impaired attachment of bacteria to PBL.

    loading  Loading Related Articles